Quantitative affinity purification mass spectrometry: a versatile technology to study protein–protein interactions
نویسندگان
چکیده
منابع مشابه
Quantitative affinity purification mass spectrometry: a versatile technology to study protein–protein interactions
While the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind. Most proteins fulfill their tasks in complexes with other proteins, and analysis of protein-protein interactions (PPIs) can therefore provide insights into protein function. Several methods can be used to generate large-s...
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Cells/tissues are then lysed and the Ab is added. Recovery of the Ab‐ t‐X complex is then done mostly using a capture resin made of ProteinA‐coated beads which bind the Fc portion of the Ab. However other systems exist which rely on other types of affinity interactions, i.e. biotinylation (strept)avidin systems or (His)6 Ni2+ columns etc. It is important to note that, several interactions (o...
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ژورنال
عنوان ژورنال: Frontiers in Genetics
سال: 2015
ISSN: 1664-8021
DOI: 10.3389/fgene.2015.00237